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KMID : 0380620150470030286
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Korean Journal of Food Science and Technology
2015 Volume.47 No. 3 p.286 ~ p.292
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Rapid Isolation Method for Preparation of Immuno-Stimulating Rhamnogalacturonans in Citrus Peels
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Lee Sue-Jung
Hong Hee-Do
Shin Kwang-Soon
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Abstract
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We developed a rapid isolation method for fractionation of polysaccharides with different characteristics, and optimized it for the polysaccharide mixture from Korean citrus peels. A crude polysaccharide mixture, citrus-peel-enzyme (CPE) fraction was isolated from the citrus peels digested with pectinase and ethanol precipitation. CPE was further fractionated with serially diluted ethanol solution (ethanol:deionized water=8:1, 4:1, 3:1, 2:1, 1.5:1, 1:1, and 0.5:1) to produce seven fractions labeled from CPE8 to CPE0.5. Fraction from CPE8 to CPE1 were mostly composed of 11 different sugars, including rhamnogalacturonan (RG) I and II, and the sugars contained arabino-ß-3,6-galactan moiety. However, CPE0.5 did not contain RG-II and arabino-ß-3,6-galactans. Treatment of macrophages with fractions CPE8-CPE1 led to a dose-dependent increase in interleukin-6 production (IL-6), while treatment with CPE1 and CPE0.5 fractions resulted in decreased levels of IL-6. These results indicate that this isolation method may be useful for the rapid fractionation of bioactive RGs from polysaccharide mixtures.
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KEYWORD
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citrus peels, immuno-stimulation, isolation method, pectin, rhamnogalacturonan I, rhamnogalacturonan II
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